Part:BBa_K271000

Aryl acylamidase

Cadmium is an exceedingly toxic heavy metal with no known biological function. At low cadmium concentrations, cells are able to adapt and resume growth after a period of stasis. Promoter yodA is only transiently activated during cadmium-induced growth inhibition. Aryl acylamidase (EC 3.5.1.13; AAA) is an enzyme that acts on the amide bond between aryl- and acyl groups. The most typical reaction is the hydrolysis of an anilide, producing a carboxylate and aniline, which is also reversible. Acetaminophen(Tyrenol) is an aryl acyl compound having amide bond is hydrolyzed by the enzyme and produce acetate and p-aminophenol. Because p-Aminophenol have red-purple color, aryl acylamidase gene will apply to reporter gene.

Compatibility

Chassis : DH5α

Plasmid : pSB3T5R

Experiments

Measure of expression by OD

http://2009.igem.org/Team:KU_Seoul/Result

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 567
  Illegal EcoRI site found at 1187
  Illegal XbaI site found at 9
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 567
  Illegal EcoRI site found at 1187
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 567
  Illegal EcoRI site found at 1187
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 567
  Illegal EcoRI site found at 1187
  Illegal XbaI site found at 9
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 567
  Illegal EcoRI site found at 1187
  Illegal XbaI site found at 9
  Illegal NgoMIV site found at 599
  Illegal NgoMIV site found at 1636
  
• 1000
  COMPATIBLE WITH RFC[1000]